Chapter 3: Observing Microorganisms Through a Microscope

1000 nm

1 micrometer (um) is equal to how many nanometers (nm)

0.001 um

1 nanometer (nm) is equal to how many micrometers (um)

Nanometer

Is the nanometer or the micrometer the smaller value?

Electron microscope

What microscope would one use to observe a virus

normal microbiota

Microbes normally present in and on the human body called - -. They help prevent growth of pathogens.

Transmission electron microscope

Which microscope can observe at the smallest mangnification

Brightfield microscope

Name the microscope used for observing microbes using simple staining techniques

Resolution

What is the term to describe the ability of the lenses to distinguish two points in fine detail and structure. Also known as resolving power

DIC

What microscope uses polarized light being split by prisms. This method is unstained

Darkfield

Which microscope uses an opaque disk placed in the condenser and creates a hollow cone. This method is unstained

Phase contrast

Which microscope brings together two sets of light rays, directed rays, and diffracted rays to form an image

Fluorescent microscope

Which microscope uses a fluorescent substance to absorb UV light and emit longer wavelength (visible) light

Confocal

Which microscope uses fluorochrome dye stains along with blue light to excite a plane of a specimen

Super-resolution light microscope

Which microscope uses two laser beams to excite microbes to glow while narrowing down the field excitement to a pin point area

Fluorescence

Which microscopy involves shining UV light so that a sample liberates visible light?

Yes

Does a brightfield microscope involve staining?

No

Does darkfield microscope involve staining

No

Does phase contrast microscope involve staining

No

Does Differential Inference Contrast microscope involve staining

Sometimes

Does fluorescence microscope involve staining

Yes, fluorochrome

Does confocal microscope involve staining

Yes, fluorescent dyes

Does a super resolution light microscope involve staining

No

Does an electron microscope involve staining?

Yes

Does a bright-field microscope use a refractive index?

Yes

Does a phase-contrast microscope use a refractive index?

Yes

Does a DIC microscope use a refractive index?

No

Does an electron microscope use a refractive index?

Yes

Does a fluorecent microscope use a refractive index?

Yes

Does a super resolution light microscope use a refractive index?

Yes

Does a confocal microscope use a refractive index?

Super resolution

Which microscope uses a STED (stimulated emission depletion)