Gel Electrophoresis

Gel electrophoresis

a technique used to separate segments of DNA based on their size using an electric current

targeting a gene with a mutation

Gel electrophoresis can be used for

agarose or polyacrylamide

What the gel tray is made of

buffer solution

What allows a current to travel through the gel tray

negative

the charge on DNA, and electrode at the end where the wells are

positive

The electrode that the DNA migrates towards

more resistance

What longer strands encounter to make them move slower through the gel so that they stay closer to the wells

visualized

Use of a dye or chemical to make the bands of DNA visible

UV light

What causes the bands to fluoresce

ethidium bromide

the chemical used to make the bands fluoresce

wells

the spaces that are loaded with DNA samples

similarities

can indicate when a genetic marker is present or not

length of DNA at a marker

The most common difference between individuals at a genetic marker

bands on the gel

DNA fragments of the same length produce