Veterinary Laboratory Procedures - Cytology Evaluation

These flashcards cover important concepts from the lecture on veterinary cytology evaluation, including microscopic slide evaluation, inflammation types, and neoplasia identification.

What categories of inflammation can be identified in cytological samples?

suppurative, granulomatous, pyogranulomatous, and eosinophilic.

what evaluation is done on 40x

differentiate cell types present and in what proportion to one another

what evaluation is done on 100x

looking for nuclear and cytoplasmic abnormalities

what are three things a cytology report should include

cell types seen, cell appearance/morphology, relative proportions

what is the term for the manifestation of aged neutrophils in fluid samples

Hypersegmented neutrophils

what does the presence of macrophages indicate

chronic inflammation

what is inflammation characterized by

relative numbers of WBC’s present

how is suppurative inflammation classified

large number of neutrophils, >85% of total nucleated cells

how is pyogranulomatous inflammation classified

significant number of macrophages present 15 - 50% macrophagees

how is granulomatous inflammation classified

>50% macrophages

what commonly manifests as pyogranulomatous or granulomatous

fungal or parasitic infections

how is eosinophilic inflammation classified

presence of >10% eosinophils in the sample

what commonly causes eosinophilic inflammation

parasitic infections, neoplastic disorders, eosinophilic granuloma complex in cats

karyolysis inflammation

rapid cell death - swollen, ragged nucleus without intact nuclear membrane

pyknosis inflammations

slow cell death (aging) small condensed nucleus

karyorrhexis inflammation

nuclear fragmentation - often follows pyknosis

what is neoplasia

homogeneous population of a single cell type

what types of cells commonly become neoplastic

epithelial cells, mesenchymal cells, discrete/round cells

what does benign neoplasia look like

cells are of the same type and relatively uniform in appearance

how do malignant cells display

cells will meet the criteria for at least three nuclear abnormal configurations

what are the abnormal nuclear configurations

anisokaryosis, pleomorphism, high or variable nucleus/cytoplasm ratio, increased mitotic activity, coarse chromatin pattern, nuclear molding, multinucleation, nucleoli that vary in size

plasma cell tumors - large numbers of cells with eccentrically location nucleus and prominent perinuclear zone

benign plasmacytomas or malignant multiple myeloma

what is called button lesions

cutaneous histocytoma