MONOCLONAL ANTIBODIES

What is the first step of making monoclonal antibodies

Inject mouse with antigen then lymphocytes produce antibodies

What is the second step of making monoclonal antibodies

Collect lymphocytes and fuse with tumor cell to make hybridoma cells

What is the third step of making monoclonal antibodies

Select single hybridoma cell producing antibody needed

What is the fourth step of making monoclonal antibodies

Allow hybridoma cell to divide by mitosis and form clones

What is the fifth step of making monoclonal antibodies

Collected and Purify monoclonal antibodies

How is monoclonal antibodies used for pregnancy tests

They are formed to detect HCG on end of test stick

How is monoclonal antibodies used for treating cancer

Make mAb w/ ability to attach to cancer antigens
Attach radioactive substance to mAbs
insert mAb into blood
mAb will then bind to antigens on cancer cells

Advantage of monoclonal antibodies

Only bind to specific cells and do not harm others

Disadvantage of monoclonal antibodies

Can produce harmful side-effects

What is good about using human lymphocytes

The body will not reject the mAbs

How is monoclonal antibodies used for detecting cancer

Attach fluorescent dye to mAbs then insert mAb into blood, mAb will then bind to cancerous cells and the fluorescent dye attaches to cells

What is a monoclonal antibody

Antibodies made from clones of a single type of cell

What are antibodies

small proteins produced by B-lymphocytes

What do antibodies do

Bind to antigens on surface of foreign material
Marks foreign material for destruction