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How to calculate 10x Dilution Factor?
20 microliter per person
+ 180 microliter dilutant
= 200 microliter sample
200 total sample/20 starting sample = 10 DF
Which grids on a hemocytometer are used for leukocytes?
the four most corners and the middle square
What is the volume of each grid square?
(1 mm)(1 mm)(0.1 mm) = 0.1 mm³
How to calculate leukocytes per volume (mm³)?
DF=10
Patient = cell count x DF = total cell count
(total cell count/0.1 mm³) x DF = leukocytes per volume mm³
How to count on grid?
count overlap on TOP only
can count either right OR left (but pick a side and indicate it)
What is the Normal Range for Leukocytes?
~8000-11000 per mm³