112 lab test 2 bacteria tests

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9 Terms

1
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<table style="min-width: 50px;"><colgroup><col style="min-width: 25px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><strong><span>Catalase test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><span>+ = fizzing, bubbling</span></span></p><p class="MsoNormal"></p><p class="MsoNormal"><span><span>- = no reaction</span></span></p></td></tr></tbody></table><p></p>

Catalase test

+ = fizzing, bubbling

- = no reaction

Hydrogen peroxide

 + = Staphylococcus aureus/epi

 

- = Streptococcus sp.

Gram positive

2
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<table style="min-width: 50px;"><colgroup><col style="min-width: 25px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 48.8pt;"><p class="MsoNormal"><span><strong><span>Coagulase test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 48.8pt;"><p class="MsoNormal"><span><span>+ = clumping, flecks of white</span></span></p><p class="MsoNormal"></p><p></p><p class="MsoNormal"><span><span>- = no clumping, may be milky</span></span></p></td></tr></tbody></table><p></p>

Coagulase test

+ = clumping, flecks of white

- = no clumping, may be milky

Rabbit plasma

+ = staphylococcus aureus

 

- = staphylococcus epidermidis

Gram positive

3
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<table style="min-width: 113px;"><colgroup><col style="width: 88px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" colwidth="88" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 48.8pt;"><p class="MsoNormal"><span><strong><span>Urease test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 48.8pt;"><p class="MsoNormal"><span><span>+ = pink colour</span></span></p><p class="MsoNormal"></p><p class="MsoNormal"></p><p class="MsoNormal"><span><span>- = stays orange</span></span></p></td></tr></tbody></table><p></p>

Urease test

+ = pink colour

- = stays orange

Phenol red

+ = Klebsiella, has enzyme urease that breaks down urea

 

Gram negative

4
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<table style="min-width: 50px;"><colgroup><col style="min-width: 25px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 48.8pt;"><p class="MsoNormal"><span><strong><span>TSI (triple sugar iron) test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 48.8pt;"><p class="MsoNormal"><span><span>+ = combos of yellow, black, red</span></span></p><p class="MsoNormal"></p><p class="MsoNormal"><span><span>- = red</span></span></p></td></tr></tbody></table><p></p>

TSI (triple sugar iron) test

+ = combos of yellow, black, red

- = red

Phenol red

+ = salmonella

 

- = no fermentation

Note: change in colour indicates fermentation

Gram negative

5
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<table style="min-width: 50px;"><colgroup><col style="min-width: 25px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><strong><span>Oxidase test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><span>+ = purple colour on paper</span></span></p><p class="MsoNormal"></p><p class="MsoNormal"><span><span>- = no purple</span></span></p></td></tr></tbody></table><p></p>

Oxidase test

+ = purple colour on paper

- = no purple

Oxidase

+ = Pseudomonas

Gram negative

6
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<table style="min-width: 50px;"><colgroup><col style="min-width: 25px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 140.35pt;"><p class="MsoNormal"><span><strong><span>Indole test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 140.35pt;"><p class="MsoNormal"><span><span>+ = pink hallow at top</span></span></p><p class="MsoNormal"></p><p class="MsoNormal"></p><p class="MsoNormal"></p><p class="MsoNormal"><span><span>- = yellow colour, no change</span></span></p></td></tr></tbody></table><p></p>

Indole test

+ = pink hallow at top

- = yellow colour, no change

Indole reagent Kovacs

+ = E. coli

Gram negative

7
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<table style="min-width: 50px;"><colgroup><col style="min-width: 25px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><strong><span>Citrate test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><span>+ = royal blue</span></span></p><p class="MsoNormal"></p><p class="MsoNormal"><span><span>- = stays green</span></span></p></td></tr></tbody></table><p></p>

Citrate test

+ = royal blue

- = stays green

Bromothymol blue

+ = Proteus 

Gram negative

8
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<table style="min-width: 50px;"><colgroup><col style="min-width: 25px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><strong><span>PAD test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><span>+ = greenish/blue colour change on the slant</span></span></p><p class="MsoNormal"></p><p class="MsoNormal"><span><span>- = remains yellow</span></span></p></td></tr></tbody></table><p></p>

PAD test

+ = greenish/blue colour change on the slant

- = remains yellow

Ferric chloride 10%

+ = Proteus

Gram negative

9
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<table style="min-width: 50px;"><colgroup><col style="min-width: 25px;"><col style="min-width: 25px;"></colgroup><tbody><tr><td colspan="1" rowspan="1" style="width: 61.4pt; border: 1pt solid windowtext; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><strong><span>String test</span></strong></span></p></td><td colspan="1" rowspan="1" style="width: 173.8pt; border-top: 1pt solid windowtext; border-right: 1pt solid windowtext; border-bottom: 1pt solid windowtext; border-image: initial; border-left: none; padding: 0cm 5.4pt; height: 46.1pt;"><p class="MsoNormal"><span><span>+ = stringy</span></span></p><p class="MsoNormal"></p><p class="MsoNormal"><span><span>- = no stringy</span></span></p></td></tr></tbody></table><p></p>

String test

+ = stringy

- = no stringy

N/A

+ = Klebsiella

Gram negative