EXPERIMENT 12: IMMUNOCHROMATOGRAPHIC RAPID ASSAY FOR DETECTING ANTI-SALMONELLA TYPHI

Stages of Typhoid fever

Incubation phase

Septicemia phase

Localization phase

The incubation phase ranges from

10-14 days

When the bacilli reach the GIT, some of the survives and attaches to the

epithelial cell of villi

This is where the bacteria multiply 

Mesenteric lymph node

In the septicemia phase, bacteria enters what organs

Gall bladder,

spleen,

liver,

bone marrow,

lungs,

kidney

Phase where secondary multiplication occur

Septicemia phase

The fully grown bacilli will be localized in what organs

Liver

Gall bladder

Spleen 

Person where S. typhi is discovered wherein she spreads the bacteria as a carrier patient

Mary Malon

Typhoid fever develops approximately _____ days after ingestion of the organism

9-14 days

During this week, the px generally experience sustained fever with prolonged bacteremia

2nd and 3rd week

Gold standard for testing

Culture

better to test in the early stage (1 week or first week of infection)

Blood culture

peak at 3 weeks, positive for Salmonella

Not usually performed because it is important to diagnose the infection earlier to the

S & S

Stool

3 weeks, same with stool but stool is higher

Urine-

Somatic O and flagellar H antigens that are the primary Ag structure used in salmonellae

Phase 1 and Phase 2 Ag

Designated as Vi Ag

Capsular (K) Ag

H Ag of salmonellae that occur only in few serotypes and determine the immunologic identity of the particular serotype

Phase 1 Flagellar Ag

Phase 1 Ag agglutinate only with

Homologous anti sera

It is a 5 minute semiquantitative colorimetric test fort typhoid fever

Tubex

Tubex detects what anti-salmonella ab from px serum

09 antibodies

Used to specifically detect soluble 09 lipopolysaccharide in an Ag-spiked buffer

Tubex

The typhidot test is expected to become positive within ____ of infection

2-3 days

The typhidot test is base on the presence of specific _____ AB

IgM and IgG