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the electron transport system is oriented in the __________ so that electrons are separated from protons
cytoplasmic membrane
what does the final carrier in the electron transport chain do
donates the electrons and protons to the terminal electron acceptor
during electron transfer, protons are released on the _____ of the membrane
outside
pH gradient and electrochemical potential across the membrane are combined to make
the proton motive force
in the pmf, what prevents the H+s from coming back into the cell
plasma membrane
where does H+ concentration increase with the PMF
extracellular fluid
PMF acts as a source of ___
energy
complex that converts PMF into ATP, has 2 components
ATPase
multiprotein extramembrane complex extending into cytoplasm
F1
membrane-integrated proton-translocating multiprotein complex, reversible catalysis of ADP + Pi to ATP
Fo
the ATPase consumes ______ H+ per ATP, ____ ATP produced per 2e-
3-4, 3
what do ATPases generate in strict fermenters
PMF for flagellar rotation and transport
total energy from 1 glucose for aerobic respiration
38
how many of the ATP from aerobic respiration are generated because of the NADH that feeds the ETC
34
when does fermentation occur
when there is no terminal electron acceptor to allow the ETC to function
fermentation provides ____ energy than respiration
less
what has to happen in fermentation to allow more glycolysis to occur and keep ATP production going
the cells need to recycle NADH back to NAD
when the cell cannot run an ETC because there is no terminal electron acceptor, they need to
recycle the NADH to NAD
in fermentation we use ___ to reduced pyruvate and this produces ___ and ferments products in the process
NADH, NAD
in aerobic respiration, ____ provides electrons and O2 is reduced to produce ___
NADH, H2O
when the O2 is the terminal electron acceptor, and generates H2O as waste
aerobic respiration
something other than O2 is the terminal electron acceptor, generates different waste products
anaerobic respiration
how is the process of using NADH to make PMF slightly different in anaerobic respiration
the O2 is not used at the end of the ETC
for anaerobic respiration in chemoorganotrophs, it still involves eating an __________ like glucose
organic compound
why is less energy released in anaerobic respiration that in aerobic
because of the E values of the acceptors compared with the O2
where is O2 on the redox tower
the very bottom
O2 is at the very ____ of the redox tower, so it is the best terminal electron ____ for allowing the release of the most _____
bottom, acceptor, energy
what type of environment is freshwater marine sediments, soils, subsurface, ect
anoxic
what are these 4 things examples of:
dissimilative nitrate reduction (denitrification)
sulfate reduction
methanogenesis
proton reduction
anaerobic respiration
what are some of the most common electron acceptors in anaerobic respiration
inorganic nitrogen compounds
______ is when you reduced NO3- to nitrite or further to nitric oxide, nitrous oxide…
denitrification
some nitrate reducers like ______ only carry out the first step of dissimilatory nitrate reduction
E. coli
______ performs nitrate reduction (NO3- →NO2-), and _____ carries out denitrification (NO3- → N2)
E. coli, Pseudomonas stutzeri
this occurs when NO3- is transformed all the way to N2, NADH provides electrons to the ETC and NO3- and subsequent reduced N compounds act as e- acceptors with eventual production and release of N2
denitrification
during denitrification, where are protons released to generate the PMF
periplasm
in nitrate reduction of anaerobic respiration, ____ provides electrons and NO3- is reduced to produce ___
NADH, NO2-
in aerobic respiration you get ___ protons per NADH, and in nitrate reduction you get ___ protons per NADH
8,6
in aerobic and anaerobic respiration in E.coli, less H+ gets pumped when using ____ because it is ____ on the redox tower than O2
NO3-, higher